![]() ![]() You can alter the slices in a stack by adding, deleting or rearranging them. When you are happy with your options, click OK to duplicate. Use these options to duplicate multiple slices or the entire stack. However, in the Duplicate window you will now have more options, where you select Duplicate Stack and can also nominate spceific slices to be duplicated intot he new stack. This can be useful if you want to extract a slingle slice from your stack for display or analysis purposes. When using Duplicate for a stack, only the current slice will be duplicated as a single image. Select your stack and follow the menu or shortcut to Duplicate as previously shown. You can use the Duplicate function for stacks also. To reverse this and generate multiple single images from a stack go to Image -> Stacks -> Stack to Images. To keep the original images select the box next to Keep Source Images. You can give the stack a new name, or keep the generic ‘Stack’. To create a stack from multiple individual images go to Image -> Stacks -> Images to Stack.Īn options window will open. You can monitor the slice number in the image information at the top left of the image window. You can also click on the left (2) and right (3) arrows to move the stack by 1 slice or click the play button (4) to run through the stack slices automatically. ![]() You can easily move through the slices in a stack using the bar at the bottom of the image.Ĭlick and drag the slider (1) to move through the stacks. The image NeuralTube.jpeg along with its individual channels and image stacks RGBStack.tif and MovieStack.tif will be used for demonstration throughout this section. More detailed informaiton on working with multiple dimmensions in imaging and stacks is covered in FIJI Intermediate. This section goes through the tools and methods for working with image stacks. You can also generate a stack yourself from related TIFF or JPEG images. When channel, Z-series or time information is recorded the images are usually opened as stacks automatically when bio-formats importer is used to open the files. Stacks can represent different channels from the same image, z-slices captured on the microscope or time series. Image stacks are composed of a number of related images opened on top of each other in a single window. ![]()
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